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CALLUS INDUCTION AND DIRECT SHOOT FORMATION IN IN VITRO CULTURED IMMATURE INFLORESCENCE TISSUES OF COCONUT

Authors:

V. R. M. VIDHANA ARACHCHI ,

COCOS, LK
About V. R. M.
Coconut Research Institute, Lunuwila, Sri Lanka.
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L. K. WEERAKOON

LK
About L. K.
Coconut Research Institute, Lunuwila, Sri Lanka.
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Abstract

Clonal propagation of elite palms is a promising possibility for producing
uniform planting material and improving productivity in coconut lands.
In the absence of a natural vegetative propagation method, in vitro culture
remains the only approach for vegetative multiplication of coconut.
Immature inflorescence material has shown to be a promising source
of explants for coconut tissue culture. In the present study, four different
culture media (medium 72; modified Eeuwens Y 3 medium; coconut anther
culture medim; modified Blake's medium) were evaluated for callus induction
in floral explants: Immature inflorescences were collected non-destructively
from 12 year old Sri Lanka-tall coconut palms and rachilla segments
obtained from these inflorescences were cultured on above media.
Out of the four culture media tested, callus production was observed
only in the explants cultured on medium 72. In this medium (which contained
24 uM 2,4-dichlorophenoxyacetic acid and 0.25% activated charcoal),
about 30% of the floral explants produced compact, highly embryogenie
calli. Direct regeneration of shoots from floral primordia occurred at &
very low frequency in the modified Eeuwens Y 3 medium which contained
200 pM 2,4-dichlorophenoxyacetic acid and 0.2% activated charcoal. No
callogenesis or direct organogenesis were observed in the floral explants
cultured on the other two culture media tested.
DOI: http://doi.org/10.4038/cocos.v12i0.2164
How to Cite: VIDHANA ARACHCHI, V.R.M. & WEERAKOON, L.K., (2010). CALLUS INDUCTION AND DIRECT SHOOT FORMATION IN IN VITRO CULTURED IMMATURE INFLORESCENCE TISSUES OF COCONUT. COCOS. 12, p.39–. DOI: http://doi.org/10.4038/cocos.v12i0.2164
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Published on 10 Aug 2010.
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