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CYTOLOGICAL EXAMINATION OF MICROSPORE DEVELOPMENT FOR MICROSPORE AND ANTHER CULTURE OF COCONUT (Cocos nucifera L.) cv SRI LANKA TALL

Author:

P. I. P. PERERA

COCOS, LK
About P. I. P.
Coconut Research Institute, Lunuwila, Sri Lanka.
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Abstract

Production of double haploids through anther and microspore culture has a
considerable potential for shortening the breeding cycle in coconut. The
developmental stage of a microspore is a critical factor in androgensis and
microspores at late uninucleate stage have been shown to be the most
suitable stage for in vitro culture for many crop species. Routine cytological
; examination of microspores for identifying the correct stage for culture is
tedious and time-consuming. In the present study, a correlation between the
microspore developmental stage and age of the spadix (in Sri Lanka Tall
coconut) in terms of weeks-before-splitting (WBS) was established for
practical convenience of anther and microspore culture. At the age of eight
WBS, anthers consisted of microspore mother cells. Within one week, these
cells underwent meiosis and produced tetrads. The release of microspores
from tetrads could be observed in six WBS spadices. Microspores at late uninucleate stage, which is reported as the most suitable stage for
androgenesis, could be obtained from three WBS spadices.
How to Cite: PERERA, P.I.P., (2010). CYTOLOGICAL EXAMINATION OF MICROSPORE DEVELOPMENT FOR MICROSPORE AND ANTHER CULTURE OF COCONUT (Cocos nucifera L.) cv SRI LANKA TALL. COCOS. 15, pp.53–59. DOI: http://doi.org/10.4038/cocos.v15i0.2188
Published on 12 Aug 2010.
Peer Reviewed

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